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Publication name: Lyons_zap1p Experiment name: DY1457 (wild type) 3 mM vs. 10 uM zinc y13-75
Red: Wild type zinc excess
Green: Wild type zinc replete
Description: Wild type (DY1457 MATa ade6 can1 his3 leu2 trp1 ura3) and zap1 (DY1457 zap1::TRP1) cells were grown in CSD (2% dextrose, 0.51% yeast nitrogen base without divalent cation or potassium phosphate 0.1g/l of adenine, histidine, tryptophan and leucine. Treated with chelex-100 ion exchange resin. Then MNSO4, FeCl3, CuSO4, CaCl2, MgSO4 and KPO4 were added) containing three different concentrations of zinc: deficient (<100nM), replete (10┬ÁM) and excess (3mM). Cells were collected by centrifugation.
Ratio min: 0.0700 Ratio max: 7.3 Average(log2ratio): 0.0 Std_dev(log2ratio): 0.3
Present: 6107 ORF - Repressed: 9(0.1%) 3 fold, 24(0.4%) 2 fold, 177(2.9%) 1.5 fold - Induced: 261(4.3%) 1.5 fold, 54(0.9%) 2 fold, 12(0.2%) 3 fold