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Publication name: Carroll_pho85 Experiment name: F82G_pho4D_1_mM_1NaPP1
Red: PHO85F82G pho4D plus DMSO 10 min
Green: PHO85 F82G pho4D plus 1 microM 1NaPP1 10min
Description: Yeast were grown overnight at 30°C in SD/complete media to an OD600. For 1-Na PP1 treatments, either 1,000× 1-Na PP1 in DMSO or an equivalent volume of DMSO was added. Cells were harvested by centrifugation, flash-frozen in liquid nitrogen, and stored at 20°C for up to 2 weeks. Total and poly(A) RNA were isolated as described, and poly(A) RNA was reverse-transcribed with StrataScript (Stratagene) incorporating amino-allyl dUTP (Sigma) at a ratio of 3:2 with dTTP
Ratio min: 0.2800 Ratio max: 4.2 Average(log2ratio): -0.0 Std_dev(log2ratio): 0.3
Present: 5995 ORF - Repressed: 2(0.0%) 3 fold, 9(0.2%) 2 fold, 208(3.5%) 1.5 fold - Induced: 140(2.3%) 1.5 fold, 22(0.4%) 2 fold, 2(0.0%) 3 fold