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/ Home / Protocols / Hybridization
Microarrays Hybridization
Yeast microarrays hybridization protocols:
 The
protocole is available in a HTML format here.
Mouse microarrays hybridization protocols:
Several
protocols are available for mouse according to the chips type used:
- For 15k and NeuroDev2 chips (PCR products), the labelling protocol is not
currently available.
- For Synapse chips (oligonucleotides), the protocol is available in a HTML
format here
- Pour les lames 22k (oligonucléotides), the protocol is available
in a HTML format here
Movie tutorials linked to microarray hybridization:
These movies aimed at help you in the most tricky stages in DNA microarray use.
All the videos on this site are in a QuickTime Movie (.mov) format. You can
freely download the QuickTime
visualization software from Apple.
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Washing steps after pre-hybridization:
In this
movie we show you a washing method using a 50ml Falcon tube. You will also see how to eliminate isopropanol excess
from the slide after the last washing step after pre-hybridization.
QuickTime movie: slide_washing_steps.mov
(
3.2 Mb )
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DNA microarray
hybridization:
This
movie show how to prepare the hybridization chamber, to deposit the
labelled cDNA target on the slide and how to cover the microarray with
a coverslip.
QuickTime movie: slide_hybridization.mov
(
1.4 Mb )
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DNA microarray overnight
incubation:
In
this movie you will see how we incubate our slides in the water bath
overnight.
QuickTime movie: slide_water_bath.mov
(
900.9 Kb )
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The first washing
step :
In
this movie you will see how to do the first washing step after water
bath microarray incubation. This stage is very important because it's
the moment where the coverslip is tearing off the slide. It is very
important when the coverslip fall out at the bottom of the Falcon tube
to turn inside out the slide to avoid any contact between the DNA spots
and the coversplip.
QuickTime movie: first_washing_step.mov
(
2.8 Mb )
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DNA microarray
centrifugation drying:
On
this movie you will see the slide drying step. The drying technique
we use rely on slide centrifugation in 96 wells microplates carrier.
QuickTime movie: slide_centrifugation.mov
(
2.9 Mb )
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DNA microarray lecture
on GenePix 4000 scanner:
This
movie aimed at explain how to correctly adjust the slide inside the
scanner. Indeed, if the slide is not correctly adjust (label on the
top), the grid will not give you the correct spot position and the result
interpretation you will be wrong.
QuickTime movie: slide_scanning.mov
(
1.2 Mb )
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